The early quantitative characterization of possible production hosts is a key element in the development of knowledge based biotechnological processes. Despite of scale independent parameters like substrate uptake rates, production rates, maximum growth rates and yields, the performance on mixed substrates, as they occur for example during induction of recombinant protein production, is of great interest. In the present work, pulse and shift experiments with glycerol, glucose, malate, pyruvate and acetate were performed in chemostat cultures of the methylotrophic yeast Pichia pastoris. This...