Interspecific crosses provide a bridge by which the gene pool of rice can be increased. Attempts have been made to visualize the genomic constitution of wide-cross derivatives. Genomic in situ hybridization Oryza australiensis chromosomes and introgressed segment from O. australiensis into the Oryza sativa background have been detected. The genomic relationship between Oryza sativa (2n=24 AA) and Oryza australinesis (2n = 24 EE) these two species was assessed by using three strategies: genomic in situ hybridization (GISH), meiotic chromosome pairing, pollen and spikelet sterility. ...