The water buffalo holds tremendous potential in livestock sector,but we are defficient in genetic data of different Pakistani buffalo breeds,which needs to be established for their genetic identification. In this study, blood samples of unrelated true representative of two breeds (Nili- Ravi,Kundi) were collected from different government livestock farms.Mitochondrial Cytochrome b gene coupled with microsattelite markers are considered to be efficient tools for breed characterization & species identification.Extraction of DNA followed by polymerase chain reactions were performed for...