More than 700 serotypes of E. coli have been identified. The different E. coli serotypes are distinguished by their "O" and "H" antigens on their bodies and flagella, respectively. The E. coli serotypes that are responsible for the numerous reports of contaminated foods and beverages are those that produce Shiga toxin, E. coli O157: H7 was first recognized as a food borne pathogen in 1982 during an investigation into an outbreak of hemorrhagic colitis (bloody diarrhea) associated with consumption of contaminated hamburgers. Present work was based on bacterial contamination of food samples...

A detailed study was undertaken in order to determine the safe dose of mustard seed meal in the diet for Japanese quails which can be used at the production level without having deleterious effects on the growth of this bird. For this purpose, some 1500 birds were fed different levels of mustard seed meal in isocaloric and isonitrogenous diets. The results of feeding of these diets for 30 days show that there are no toxic effects on the growth of birds. In fact the birds on the experimental rations were either comparable or heavier than the control except 5% group. Different biochemical and...

A minority of Escherichia coli (E. coli) strains are capable of causing human illness. E. coli O157: H7 has now been reported from Pakistan. Infection with E. coli O157: H7 may present with a wide range of clinical manifestations. This research work was based on Microbial, Immunological screening and Molecular study of pathogenic bacteria E. coli in meat samples (Beef, Mutton, Quail and Fish), in hawkers foods: dahi bhala, fruit chat, fruit juices and milk shakes. 300 Meat samples, fruit juices and milk shakes were collected from Lahore. 300 Dahi bhala & fruit chat samples were collected from...

Pathogenic Escherichia coli are found in the contaminated raw meat. Contamination is common in Pakistan due to unhygienic condition. The present work was based on the entomopathogenic effect of E.coli against Tribolium castaneum. The cultured sample of E.coli O157 isolated from raw meat was used in this study. Microbial screening of pathogenic bacteria was done by using different media such as TSB, MacConkey and Novobiocin. Rearing of Tribolium castaneum was done in the Laboratory of LCWU. Bioassay technique was applied for the detection of effect of E.coli against the Tribolium castaneum....

Pathogenic Escherichia coli is found in the contaminated raw meat, raw milk, raw vegetables, contaminated water. The present work was based on the entomopathogenic effect of E.coli against stored grain pest Tribolium castaneum. Microbial screening of pathogenic bacteria was done by using different media such as TSB media, MacConkey media and Novobiocin media. Rearing of Tribolium castaneum was done and different stages of Tribolium castaneum such as larvae, pupae and adult were observed. Bioassay technique was applied for the detection of effect of E.coli against the Tribolium castaneum. The...

Microbial control of insect pest of crops using entomopathogens is pest management strategy. Bacillus thuringiensis has a great potential for this purpose. The focus of the present study was to determine the individual and combined effect of different insecticides such as Saraca indica as a botanical insecticide, B. thuringiensis and chemical insecticides against larvae and adults of T. castaneum. Results were analyzed by SPSS probit analysis, LC50 was noted for all these insecticides and results were compared with each other. According to the results from the individual insecticide B....