Lactating Nili-Ravi Buffaloes were injected rbST (500 mg/16 day) to observe effect on milk production, composition, physiological profile, minerals of serum, milk and mammary health biomarkers. Respiration rate was significantly high after day 1 of first injection and low during days 24, 28 and 32 of second injection irrespective of their groups. Overall mean ESR, neutrophils percent decreased and lymphocytes percent increased significantly in bST treated as compared to control buffaloes. The overall increase in milk production was 9.31% from bST treated buffaloes. Overall mean milk lactose...