This book consists of different DNA manipulation methods (BioBrick, SLIC, CPEC, Golden Gate, GoldenBraid, MoClo, USER etc.) assembly techniques (MAGE and CAGE), genetic regulations (modifying ribosomal parts, carboxysomal subunits) via modifying numerous metabolic pathways (Mevalonate pathway, Glucaric acid pathway etc.) leading to recreation of cells or artificial cellular structures. It have immense hope for future of mankind by different application in biofuel productions, cancer therapy, bioremediation, crop engineering, biodegradation etc.