The Handbook of Plant Ecophysiology Techniques you have now in your hands is the result of several combined events and efforts. The birth of this handbook can be traced as far as 1997, when our Plant Ecophysiology lab at the University of Vigo hosted a practical course on Plant Ecophysiology Techniques. That course showed us how much useful a handbook presenting a bunch of techniques would be for the scientists beginning to work on Plant Ecophysiology. In fact, we wrote a short handbook explaining the basics of the techniques taught in that 1997 course: Flow cytometry to measure ploidy...