A simple and rapid HPLC assay procedure has been developed and validated for the quantitative determination of metronidazole benzoate from commercially available preparations. The HPLC system was developed based on the BP 2007 monograph of metronidazole suspension. However the various parameters were modified according to column and equipment available. The HPLC conditions used were, column: C18, detection: UV at 254 nm; injection volume: 20 l; mobile phase: methanol and de-ionized water (13:8, v/v); flow rate 1.0 ml min 1. The procedure separated metronidazole benzoate, in an overall...