Over the last decade, the applications of
RNAi and microarray have increased exponentially, and are
being applied to explore the expression profile of whole
genome (particularly genes coding for proteins). Very
little literature exists regarding RNAi against non-coding
RNA (hot stuff nowadays) and the expression profile of
non-coding RNAs. This book therefore, provide a systematic
approach of targeting all types of non-coding RNAs
(snoRNAs, snRNAs, SRP and Y RNA) by RNAi and expression
...

In earlier days, production of industrially important enzymes was carried out by conventional methods of optimizing cultural conditions in flasks and fermentors. With passage of time new techniques are being developed like cloning. In cloning specific genes are amplified and ligated in specific plasmid that is in turn transformed into the host cells. In this book cloning and expression of alpha amylase gene of Bacillus licheniformis is discussed. pTZ57R/T and pET22b plasmids are used to clone amylase gene. Reasonable amount of amylase is obtained. By using this technique large amount of...

In the past 50 years, numerous polypeptide antibiotics have been identified mainly by bacterial production. These polypeptide antibiotics have been shown to have antibacterial activity against many bacteria specially gram positive bacteria. Some of these polypeptide antibiotics could also be used as growth promoter in poultry feed. This book also provides detailed information about production of bacitracin by Bacillus licheniformis. In this regard, systematic approach was adopted. In the first step, different isolates of Bacillus licheniformis were isolated from soil, milk and poultry...

In earlier days, production of industrially important enzymes was carried out by conventional methods of optimizing cultural conditions in flasks and fermentors. With passage of time new techniques are being developed like cloning. In cloning specific genes are amplified and ligated in specific plasmid that is in turn transformed into the host cells. In this book cloning and expression of xylanase gene of Bacillus licheniformis is discussed. pTZ57R/T and pET22b plasmids are used to clone xylanase gene. Reasonable amount of xylanase is obtained. By using this technique large amount of enzyme...

Since the discovery of microRNAs (miRNAs) their role has now been established in translational repression and gene silencing. In metazoans the miRNA target sites are in the three prime untranslated regions (3'UTR) of the mRNA. The function of miRNAs appears to be in gene regulation. Several miRNAs have been found to have links with some types of cancers, heart diseases, nervous system etc. In this book specific sites targeted by miRNAs in the 3 UTRs of mRNAs in the genome of C. briggsae were identified with the help of thermodynamic studies. RNAhybrid software was also utilized in the...

This book deals with cloning of maltase gene of Bacillus licheniformis in Escherichia coli. During the course of study, genomic DNA isolation from Bacillus licheniformis strain ATCC 14580 has been followed by the amplification of maltase gene using a pair of specific forward and reverse primers under optimized PCR parameters. The DNA isolated from Bacillus licheniformis was used as a template and followed amplification, PCR product was purified using Novagen gene kit. T4 DNA ligase was used to ligate the purified PCR product with cloning vector pTZ57R/T. Escherichia coli strain DH5 was used...