Drosophila is an emerging model for studying genetic influences on heart function, and has also been found to be highly tolerant to hypoxia. We adopted a systems biology approach to discover important hypoxia-tolerance strategies in ATP-generating metabolism in Drosophila heart. This work describes the design of automation technology for rapidly screening the in vivo cardiac hypoxia response in adult flies, the use of NMR metabolomics to gather global metabolic profiles, and the integration of these data into a genome-wide reconstruction of central metabolism. The model was further...