Chromatographic results from four, neutral steroids highlight the repeatable chromatography achieved on Spherisorb ODS1 phase and the non-repeatable chromatography expressed on Spherisorb ODS2 phase, with respect to retention, resolution and analyte selectivity of three keto steroids and ethinyl estradiol, a phenolic steroid. The significant differences between the two phases are that ODS2 contains a higher percent carbon load and ligand coverage, as a result of endcapping. A noteworthy and significant property of the silica component used as substrate, which is known as "type A" silica,...