This volume contains the proceedings of the 16th International SPIN Workshop on Model Checking of Software (SPIN 2009), that was held at the Grenoble World Trade Center, in Grenoble, France, June 26 28, 2009. The workshop was co-located with the 21st International Conference on Computer-Aided Veri?- tion (CAV 2009). The SPIN workshopis a forum for practitioners and researchersinterested in themodelchecking-basedanalysisofsoftwaresystems.Thefocusoftheworkshop is on theoretical advances and empirical evaluations related to state-space and path exploration techniques, as implemented in the SPIN...