In the pharmaceutical industry mammalian cell lines are the primary expression system in the production of therapeutic recombinant proteins. Despite its diverse drawbacks, the stirred tank reactor serves here as major workhorse for most applications. To overcome the limitations faced, improved bioreactors are needed to provide a more favorable in vitro environment to mimic in vivo conditions. In order to establish a suitable culture system, the cells responsiveness to environmental stress is evaluated to identify and anticipate the impact of this process parameter during cell cultivation....