Agrobacterium tumefaciens causes crown gall disease
on plants and is the primary vector in genetic
engineering of plants. Agrobacterium transports DNA
and proteins to the plant cell via a Type IV
Secretion System. This system is similar to secretion
systems in human pathogens and those used for DNA
exchange during bacterial conjugation. To better
understand this process, a genetic screen using yeast
as the genetically engineered host was developed to
identify host proteins involved in transformation.
While developing this screen, factors that alter
transformation of yeast were identified. The...