PCR, developed at Cetus Corporation/USA by Henry A. Erlich, Kary Mullis and Randall K. Saiki, is a very simple method for amplifying nucleic acids in vitro. The realization of this idea bases on the repetition of a set of three different temperatures and yields an increase of the target structure up to a factor of 106 to 1012. Therefore, this technique is predisposed for safe analysis and characterization of DNA and RNA sequences of interest, even where the starting amount of material is enormously small. Because of its sensitivity, speed and versatility this method is particularly suitable...

Friedrich Adolph Krummacher war ein evangelischer Theologe und zugleich ein Bestsellerautor der Goethezeit. Eine Vielzahl von ausserordentlichen Dokumenten ermoglicht es, ein ganz nahes und lebendiges Bild von ihm zu gewinnen und mittels seiner Bekanntschaft in eine Zeit einzutauchen, in der uns manches fremd und vorgestrig, vieles aber auch uberraschend nah und zuganglich erscheint. Das Buch konzentriert sich dabei auf die Kettwiger Jahre, 1807 - 1812; eine historische Ausnahmesituation, die Franzosenzeit" unter Napoleon. Wie erlebt der Dichter, Pfarrer, Freund und Familienvater diese Zeit?...

Jane Austen's Pride and Prejudice is one the most widely read and critically acclaimed novels in the English language. It was first published in England in 1813, and later in America, in 1832, under the name, Elizabeth Bennet or, Pride and Prejudice. It has since been translated into French, German, Danish and Swedish. This essay examines various characters in Jane Austen's novel, and proposes to explain why Faith, Hope, and Love are the key Christian virtues being explored internally the author.

How far from your native country would you travel to find a place to live, in freedom? What if it meant leaving behind everything and everyone you had ever loved, if only because of a slim chance that a better life might exist someplace else? Book One of Land of the Free, Home of the Brave is the story of a thirty-two year-old Italian stonemason who had such a dream, as well as the faith and the courage to make it come true, both for himself and for his family. Pasquale DeVito was a man with no money, education, or connections. One day, while walking through his village, he saw a poster; and...

Fate... Free Will... Destiny... Predestination. What do these all have in common? Do human beings actually have free will, or does God and Nature have a say in the matter? This novel attempts to answer those questions for one person in particular. Richard Desmond lived at the end of the 20th Century and was both an accountant and a poet. When he was forty-eight years old he lost his job but found true love. His life's portrait was composed of a series of choices; whether they were good or bad, readers must decide for themselves.

Book Two of Land of the Free, Home of the Brave continues the saga of the DeVito family as they attempt to gain a foothold in their newly-adopted country. Michael DeVito hated the English language but knew that he must master it, if he wanted to follow his dreams. Most of all, he wanted to become a great actor, but he was forced to drop out of school to support his family; and the tailoring factory where he worked was a dangerous, dehumanizing, and unsanitary place. Bernard Hillman was a union organizer, and he and Michael DeVito found a way to force the factory owners to the bargaining...

Book Three of Land of the Free, Home of the Brave continues the saga of the DeVito family as they attempt to reconcile their Italian heritage with the opportunities and demands presented by the dominant American culture. Mary DeVito was born in America but found herself trying to live between two different worlds: the one belonging to her Italian-American parents, and the other belonging to the American culture of the thirties, forties and fifties. Throughout high school, Mary learns to meet the challenges placed upon her by the beginning of World War II. Later, she decides to go to college...

Book Four of Land of the Free, Home of the Brave completes the saga of the DeVito family in America and concerns the great-grandson of the stonemason who originally emigrated from Italy to the United States in 1898. Jay Marks lives in San Diego, California and works as a journalist for the San Diego Sun. The newspaper is flying him over to Europe to cover the biggest earthquake to hit Southern Italy in over a hundred years. In Rome, Jay runs into Howard Connors, a cameraman and long-time friend. In Naples, Jay and Howard meet Laura LaBianca, a nurse working with the Red Cross relief action....