Methods for Obtaining X-Ray Diffraction Patterns from Drosophila 198 Diffraction Patterns from Drosophila IFM 203 Concluding Remarks 211 Note Added in Proof 211 17. Functional and Ecological Effects of Isoform Variation in Insect Flight Muscle 214 James H. Marden Abstract 214 Introduction 215 Nature's Versatile Engine 215 The Underlying Genetics: An Underinflated Genome and a Hyperinflated Transcriptome and Proteome 216 Functional Effects of Isoform Variation 219 Alternative Splicing and the Generation of Combinatorial Complexity 220 Functional Consequences of Naturally Occurring Isoform...