Fluorescence spectroscopy has traditionally found wide application in bio chemistry and cell biology. Since there are relatively few naturally occurring fluorescent biomolecules, fluorescence spectroscopy offers a combination of great specificity and sensitivity. Historically, these features have been ex ploited with great success utilizing both intrinsic and extrinsic probes. Re cent applications have built upon these traditional strengths and have re sulted in the development of new instrumental techniques, novel and convenient fluorescent probes, and a deeper, theoretical understanding of...

Fluorescence spectroscopy has traditionally found wide application in bio- chemistry and cell biology. Since there are relatively few naturally occurring fluorescent biomolecules, fluorescence spectroscopy offers a combination of great specificity and sensitivity. Historically, these features have been ex- ploited with great success utilizing both intrinsic and extrinsic probes. Re- cent applications have built upon these traditional strengths and have re- sulted in the development of new instrumental techniques, novel and convenient fluorescent probes, and a deeper, theoretical understanding...