This book starts with an overview and introduction on the trends in nanofabrication and nanoimprint technology, followed by a detailed discussion on the design, fabrication, and evaluation of nanoimprint biosensors. The proto-model systems and some application examples of this sensor are also included in the chapters. The book will appeal to anyone in the field of nanotechnology, especially nanofabrication, nanophotonics, and nanobiology, or biosensor research.