A collection of readily reproducible methods for the design, preparation, and use of RNAs for silencing gene expression in cells and organisms. The techniques range widely and include methods addressing the biochemical aspects of the silencing machinery, RNA silencing in non-mammalian organisms, and the in vivo delivery of siRNAs and silencing vectors. There are also techniques for designing, preparing, and using RNAs to silence gene expression, for fine-tuning regulation by targeting specific isoforms of a given gene, and for the study and use of microRNAs. The protocols follow the...

The Tampakan Highlands of the Central
Mindanao Cordillera in the Philippines have received
international attention due to the discovery of a
copper-gold deposit in the 1990s and subsequent minerals
exploration and community development initiatives as part
of the Tampakan Project. This book compares infant and
child mortality parameters of indigenous Bla-an and
Christian Visayan populations residing within the project
area over a fifteen-year period (1983-97). In so doing, it
...

A collection of readily reproducible methods for the design, preparation, and use of RNAs for silencing gene expression in cells and organisms. The techniques range widely and include methods addressing the biochemical aspects of the silencing machinery, RNA silencing in non-mammalian organisms, and the in vivo delivery of siRNAs and silencing vectors. There are also techniques for designing, preparing, and using RNAs to silence gene expression, for fine-tuning regulation by targeting specific isoforms of a given gene, and for the study and use of microRNAs. The protocols follow the...