This book presents a novel technique of delivering membrane impermeable molecules into single living cells with the assistance of femtosecond (fs) near-infrared (NIR) laser pulses. This technique promises several major advantages, namely, very high transfection efficiency, high cell survival rate ( 100%) and fully preserved cell viabilities. In this work, the system for fs NIR opto-injection was designed and built. Successful fs NIR opto-injection has been performed on several cell systems including single mammalian cells, marine animal eggs, and human cancer cells cultured in a tissue-like...