Remote loading of liposomes by transmembrane gradients is one of the best approaches for achieving the high drug level per liposome. This breakthrough, which enabled the clinical use of nanoliposomal drugs, has not been paralleled by good understanding that allows predicting loading efficiency of drugs. We developed a model that relates drug physicochemical properties and loading conditions to loading efficiency. This model enables choosing candidate molecules for remote loading and optimizing loading conditions according to logical considerations. In order to improve the therapeutic...