Cell Culture Methods for in vitro Toxicology introduces the reader to a range of techniques involved in the use of in vitro cell culture in toxicological studies. It deals with major cell types studied in the field of toxicology and will be useful for anyone wishing to start work with animal cell cultures or to refresh their knowledge relating to in vitro cell models. Fundamental chapters deal with the general biology of cytotoxicity and cell immortalisation these are key issues for in vitro systems addressing the 3Rs' principle. Up-to-date overviews deal...

Cryopreservation and Freeze-Drying Protocols: Second Edition is a compilation of robust, reproducible techniques for the conservation of a wide range of biological materials. This widely expanded second edition includes novel approaches and protocols for biological materials that were not preservable when the first edition was published in 1995.

This volume begins with a discussion of long term ex situ conservation of biological resources, the role of biological resource centers, and fundamental principles of freeze-drying and cryopreservation. Each chapter focuses on the...

There has been a growing awareness in recent years of the need for safe and regulated handling of biological materials in order to prevent health hazards and environmental contamination. Although individual governments have their own regulations, there is no comprehensive guide to safe laboratory practice. This book provides practical information for the routine handling of cell and tissue culture, giving guidelines on the setting up of safe laboratory practice and details of the national and international regulations covering the safe handling of biological materials. The book should be...