This monograph describes the theoretical bases and experimental prerequisites for methods such as spin fluorescence, triplet, Mö ssbauer, photochromic and electron-density labeling, including the procedures used to obtain specifically modified proteins, enzymes, biomembranes, nucleic acids, and other biological molecules. The fundamentals of the physical theory behind each technique is explained and details are given of how to interpret the experimental data obtained. Special sections deal with critical reviews of recent data on the structure, molecular dynamics and conformational...

Enzyme catalysis is an important and vigorously developing field of basic and applied research, posing challenging problems to biochemists and chemists. This volume embraces modern areas of enzyme catalysis where other books in the field concentrate mainly on kinetic, bioorganic and biochemical aspects of the enzyme catalysis and do not cover biophysical and physicochemical problems. Topics covered include: modern physical and kinetic methods of investigation;contemporary theories of elementary chemical processes in enzymes; structure, dynamics and action mechanism of enzyme active sites;...