In the context of forest sustainability, this book presents the issues related to both global climate change and conservation of biodiversity. It highlights four methodologies and shows how they contribute in overcoming the ecological challenges facing our world. The practical experience presented can be applied to the implementation of successful sustainable forestry policies.

Gene Targeting and Embryonic Stem Cells is a practical guide designed for the rapidly growing number of researchers who are moving into this field.

Provides details on how to culture, transfect and differentiate established cell lines, and how to isolate new cell lines. Gene targeting experiments are described for a number of cell types, including ungulate fetal fibroblasts, murine ES cells, human embryonal carinoma cells and human ES cells, and include protocols for gene-targeting vectors, DNA transfection and RNA interference.