Until the time she is eleven years old, Marie Hamilton lives a peaceful life with her grandmother, parents and younger brother on the family estate in England; however, her life changes dramatically when her family moves to France just before the start of the French Revolution. Marie's father is returning to France at the invitation of Louis XVI to reclaim his title and estates as the Comte de Grandville. The family arrives at the magnificent palace of Versailles and quickly becomes part of privileged court life. All is not well, though, in France and only months later the oppressed and...

The first libraries of complementary DNA (cDNA) clones were con structed in the mid-to-late 1970s using RNA-dependent DNA polymerase (reverse transcriptase) to convert poly A* mRNA into double-stranded cDNA suitable for insertion into prokaryotic vectors. Since then cDNA technology has become a fundamental tool for the molecular biologist and at the same time some very significant advances have occurred in the methods for con structing and screening cDNA libraries. It is not the aim of cDNA Library Protocols to give a comprehensive review of all cDNA library-based methodologies; instead we...

It must be allowed that Mrs. Desmond, with the best dispositions in the world towards children in general and her most perplexing little stepdaughter Helen in particular, was not very happy in her method of dealing with young people. Brought up herself by two maiden aunts on the old-fashioned repressive system, from which she had never consciously suffered, the children of to-day, with their eager, uncontrolled impulses, their passionate likes and dislikes, often fostered by their elders, and their too early developed individualities, were simply a painful enigma to her.