In situ hybridization is a technique that allows for the visualization of specific DNA and RNA sequences in individual cells, and is an especially important method for studying nucleic acids in heterogeneous cell populations. in situ Hybridization in Electron Microscopy reviews the three main methods developed for the ultrastructural visualization of genes:
hybridization on ultrathin sections of tissue embedded in hydrophilic resin (post-embedding method)
hybridization prior to embedding (pre-embedding)
hybridization on ultrathin sections of frozen tissue (frozen tissue method)....