A detailed methodology for analysis of various lipid signalling pathways. Authoritative contributors explain the factors that regulate lipid second messenger production by agonist-activated enzymes and examine their products. Topics discussed include procedures used to measure lipid-derived mediators such as lysophospholipids, arachidonic acid, eicosanoids, anandamide and ceramides, and the enzymes responsible for generating these messengers, such as phospholipases, prostaglandin endoperoxide synthases and sphingomyelinase.