The contributors have been selected for their prominence in their spec ific fields and because they run laboratories that actively collaborat e with other scientists. Researchers and practitioners, both beginners and experienced, who are looking for new ideas and who are interested in applying these more advanced methods will be assisted in their wor k by these commentaries. This guide provides hands-on information to complement theoretical understanding. The theory behind these methods can be found in existing books and in the original literature. However, no other guide will help you make a...

Antibodies protect us from a wide range of infectious diseases and cancers and have become an indispensable tool in science both for conventional immune response research as well as other areas related to protein identification analysis. This second edition of Making and Using Antibodies: A Practical Handbook provides clear guidance on all aspects of how to make and use antibodies for research along with their commercial and industrial applications.

Keeping pace with new developments in this area, all chapters in this new edition have been revised, updated, or...

This volume provides an overview of a variety of approaches to biological image analysis, which allow for the study of living organisms at all levels of complexity and organization. These organisms range from individual macromolecules to subcellular and cellular volumes, tissues and microbial communities. Such a "systems biology" understanding of life requires the combination of a variety of imaging techniques, and with it an in-depth understanding of their respective strengths and limitations, as well as their intersection with other techniques. Howard, Brown, and Auer show us that the...