Divided into six chapters, this guide covers basic concepts of HPLC; instrumentation; stationary phase materials; eluents; column efficiency; and the influence of physical chemistry on separations.

This book is a contemporary review of selected subjects in liquid chromatography, especially of the technical development, rather than the applications. The subjects are focused in the biomedical and environmental fields. This is also a troubleshooting record. Complex analytical problems such as sensitivity (sensitive detection by chemiluminescence, coulometric detection, laser-based detection, necessity of degassing the system for sensitive detection), difficulty (free radical detection by Electron Spin Resonance, Polarimeter for chiral recognition) and reproducibility (packings for chiral...