This book is intended to be a working guide to the operation of capillary electrophoresis (CE) instrumentation. Since CE is still a rap idly maturing technique, detailed validated protocols are not widely established. Therefore, extensive experimental procedures are not pro vided for individual analyses. The intention is to provide general guide lines on the principles and practice of CE and to give an overview of the specific technologies and important application areas. Part I provides operating instructions for standard commercially available instruments. Guidelines are included for...

This book is intended to be a working guide to the operation of capillary electrophoresis (CE) instrumentation. Since CE is still a rap idly maturing technique, detailed validated protocols are not widely established. Therefore, extensive experimental procedures are not pro vided for individual analyses. The intention is to provide general guide lines on the principles and practice of CE and to give an overview of the specific technologies and important application areas. Part I provides operating instructions for standard commercially available instruments. Guidelines are included for...

Die Kapillarelektrophorese (CE) ist heute in biochemischen Laboratorien noch nicht als Routinemethode zur DNA-Trennung etabliert, obwohl sie diverse Vorzuge gegenuber den derzeitigen Methoden besitzt.
In diesem Band stellen verschiedene Wissenschaftler die theoretischen Aspekte der Trennung von Oligonucleinsauren mit der CE, die instrumentellen Grundlagen und deren Grenzen und schlielich die praktische Anwendung in der Biochemie und Molekularbiologie vor.

This book on capillary electrophoresis is unique in its focus on the separation of nucleic acids. The importance of...

During the 1980's the analysis of pharmaceuticals was dominated by the use of High Perfor mance Liquid Chromatography (HPLC). Other separative techniques such as Gas Chromato graphy and Thin Layer Chromatography offered alternatives but their quantitative capabili ties and/or solute range could not approach that of HPLC. The majority of pharmaceuticals are ionic and it would be reasonable to assume that electrophoresis may be useful in the analysis of pharmaceuticals. However, the electrophore tic instruments available in the 1980's were labour intensive and employed post-separation detection...

Carbohydrates are ubiquitous. They come as monomers, oligomers and polymers with a startling variety of chemical, physical and material properties. They affect almost every as pect of human live. For example they are an essential part of our nutrition. Cellulose, a car bohydrate polymer, acts as a central material in civilization: as building material for housing and as paper for written communication. Scientifically, the interest in carbohydrates ranges from structural elucidation of simple carbohydrates to fundamental biochemical processes such as photosynthesis and glycolysis. Always, a...

The goal of this book is to show recent developments in the CE analysis of protein pharmaceuticals. It is devoted completely to practical concerns to strengthen the use of CE within the biotechnology industry, highlighting the uses of CE in various areas of product development including formulation studies, process development, product characterization and validated lot release and stability testing.