Culture of Specialized Cells

Culture of Immortalized Cells

Edited by R. Ian Freshney and Mary G. Freshney

The ability to develop immortalized cells in vitro is a powerful tool for the biological investigator. Most cells can only be reproduced unchanged for a limited number of cell generations before the initial cell lines die. The immortalization of cells establishes continuous cell lines, playing an important role in the study of the biology of cell growth, differentiation, and senescence. It also increases our understanding of aberrations in...

This comprehensive resource provides stepwise protocols for the in vitro cultivation of the major types of human tumors. Written in a reader-friendly style, this book is organized by specific tumor, discussing cultures for lung, gastric, colorectal, pancreas, bladder, prostate, ovary, cervix, mammary carcinoma, myoepithelium, squamous, melanoma, lymphoma, glioma, and neuroendocrine tumors. Detailed techniques for initiation, propagation, and characterization of established cell lines are presented. Drug treatment, selection, differentiation, assays for malignant cells, risks, and applications...

Step-by-step, practical guidance for the acquisition, manipulation, and use of cell sources for tissue engineering

Tissue engineering is a multidisciplinary field incorporating the principles of biology, chemistry, engineering, and medicine to create biological substitutes of native tissues for scientific research or clinical use. Specific applications of this technology include studies of tissue development and function, investigating drug response, and tissue repair and replacement. This area is rapidly becoming one of the most promising treatment options for patients suffering from...