The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.

The methods presented in this volume will enable the reader to design effective strategies for the expression of cloned genes and cDNAs and will prove useful in solving the majority of expression problems one is likely to encounter.

The Cumulative Subject Index for Volumes 195(198 and 200(227 includes the contents of the volumes covered, the subject index, contributor index, and a complete list of all the volumes published in the series. This volume supplements the Methods in Enzymology Cumulative Index Volumes 33, 75, 95, 120, 140, 175, and 199.

This volume presents methods related to the role of anaerobic dissimilatory cycles and sulfur metabolism.

• Sulfate Transport
• Dissimilatory Sulfate Metabolism
• Dehydrogenases
• Hydrogenases from sulfate-reducing bacteria
• Electron carrier protein sulfate-reducing bacteria
• Terminal reductases of sulfate-reducing bacteria molecular biology
• Dissimilatory sulfur reduction
• Oxidation of reduction compounds
• Metabolism of polythinates
• Special techniques

General Description of the Volume:
This volume, as do the other Enzyme Kinetics and Mechanism volumes in the Methods in Enzymology series, provides treatment of dynamic and chemical approaches for investigating enzyme catalysis and regulation, as well as designing metabolic inhibitors. It will greatly interest those involved in enzyme chemistry, metabolic control, and drug design. It should also interest those developing commercial applications for enzymes whose properties have been re-engineered using recombinant DNA technology and site-directed...

The critically acclaimed laboratory standard for forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerlyawaited, frequently consulted, and praised by researchers and reviewers alike. More than 250 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences.

• Methods for the quantitation of apolipoproteins related to cardiovascular disease
• Problems...

This volume of Methods in Enzymology and its companion volumes 280, 281, and 282 present new methods and their modifications developed in the past decade plus the more recently acquired knowledge on the functional and metabolic aspects of vitamins and coenzymes. They represent the only in-depth treatment dealing with methods related to vitamins and coenzymes, and contain methods that have never been grouped together before.

• This volume and its companion Volumes 280, 281, and 282 provide
• A collation of the most recent and useful methods for the identification,...

This cumulative subject index for Methods in Enzymology covers volumes 263, 264, and 266-289. In addition to the Subject Index, this volume includes the complete contents of each volume indexed and a Contributor's Index.

Chemokines are major mediators of immune cells and are involved in a wide range of proinflammatory human diseases, including rheumatoid arthritis, multiple sclerosis, and organ transplant rejection. It has recently been discovered that their receptors are involved in HIV infection. The characterization of these molecules and their receptors is thus of primary importance in understanding a number of human diseases and infections. This volume and its companion Volume 288 on Chemokine Receptors provide comprehensive experimental protocols used in this field of research....

General Description of the Volume:
This is the Cumulative Subject Index for Methods in Enzymology Volumes 290-319. It will supplement the Methods in Enzymology Index Volumes 75, 95, 120, 140, 175, 199,299, 265, and 285. In addition to the Subject Index, this volume includes the complete Contents of each volume indexed, and a Contributor's Index.
General Description of the Series:
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of...