Like other biomedical sciences, medical virology has undergone a revolution of diagnostic and scientific approaches through the advent of molecular biological techniques. Developing and maintaining an appropriate mixture of classical and molecular techniques for viral analysis is one of the challenges of medical virology today, and this volume addresses these issues. Topics covered include a broad description of "classical" techniques in viral diagnosis, nucleic acid detection by extraction and hybridization, use of the polymerase chain reaction, the application of various molecular...

The involvement of carbohydrates in biological processes has greatly fuelled the current interest in this diverse range of molecules. This book has been produced in order to answer the need for a handbook of up-to-date laboratory protocols in this field. It gives details of the approach needed to analyze a wide variety of carbohydrates and carbohydrate-containing molecules, such as polysaccharides, glycoproteins, and glycolipids, and shows how particular analytical problems should be tackled. This second edition of a very successful title in the Practical Approach series covers the new...

Neural Cell Culture brings together methods for preparing cultures fro m a diverse range of neural tissues and cells, from both the central a nd peripheral nervous systems of mammalian and avian species. Importan t features of this volume are the clearly illustrated dissection guide s accompanying each chapter, methods for enriching and purifying disti nct cell types, and guidance on optimizing cell viability and growth. Each chapter includes valuable step-by-step protocols, hints and tips for success, advice, and background information.