This volume provides detailed laboratory protocols, advice, hints and tips, example data, key literature citations, background information, and troubleshooting comments for the steps used in most molecular biology laboratories for example transformation, construction of cDNA and genomic libraries, probe preparation, analysis of gene organization and expression, in vitro mutagenesis, and DNA sequencing. The information provided is an completely up-to-date account of each topic by established researchers.
These two books, the first of four volumes are thoroughly revised and up-dated...

Expression systems are in widespread use for cloning specific genes, for synthesizing the encoded proteins for structural and functional analysis, and for large-scale preparation of current use with background information and advice on the merits of each, step-by-step practical protocols, troubleshooting, and details of the latest applications. These two books, the first of four volumes are thoroughly revised and up-dated versions of the original ones published in the Practical Approach series. Together they will offer a complete guide to the major techniques required by modern molecular...

Drosophila--the scientist's favorite laboratory organism--is a key sub ject for studying genetics, development, and behavior. The second edit ion of this popular hands-on guide brings the methodology up-to-date, with contributions from the leading researchers in the field.

Crystallography is the major method of determining structures of biological macromolecules yet crystallization techniques are still regarded as difficult to perform. This new edition of Crystallization of Nucleic Acids and Proteins: A Practical Approach continues in the vein of the first edition by providing a detailed and rational guide to producing crystals of proteins and nucleic acids of sufficient quantity and quality for diffraction studies. It has been thoroughly updated to include all the major new techniques such as the uses of molecular biology in structural biology (maximizing...

Written and edited by experts in the field, this book provides valuabl e insight into the remarkable advancement of DNA microarrays. It is es sential for researchers investigating patterns of gene expression and useful for all those interested in the use of biochips.

This new edition of Animal Cell Culture covers new or updated chapters on cell authentication, serum-free culture, apoptosis assays, FISH, g enetic modification, scale-up, stem cell assays, 3-dimensional culture, tissue engineering and cytotoxicity assays. Detailed protocols for a wide variety of methods provide the core of each chapter, making new methodology easily accessible. Everyone working in biological and medi cal research, whether in academia or a commercial organization, practi sing cell culture will benefit greatly from this book.

Provides an introduction to the use of radioactivity in the bioscience laboratory. The text covers general aspects of radioactivity, methods for the detection of radioactivity, radioisotope protocols used to study key cellular processes, and a summary of legislative requirements in the US and European Union. Guidance on safe handling and detailed recipes are provided.

With the 'post genomics' era comes an increasing demand for the techniques of cell biology, critical to interpreting the function and location of the cell's myriad proteins and macromolecules. In response, this second edition of Plant Cell Biology balances established techniques, including classical histochemistry and electron microscopy, with new developments in the field. The book covers a substantial range of methods for working on living cells, including the application of fluorescent probes, cytometry, expression systems, the use of green fluorescent protein, micromanipulation and...