Attempts to transfect the prion protein in human cancer cell lines

ISBN-13: 9783845442075 / Angielski / Miękka / 108 str.

Khor Heng Wei; Yeoh Bee Yoong; Toh Li Fang

Opis

Transmissible spongiform encephalopathies (TSE) or prion diseases are a group of fatal neurodegenerative diseases that affects humans and animals. The characteristic feature of prion diseases is conversion of normal cellular prion (PrPc) to the abnormal pathogenic scrapie isoform (PrPsc) through a still undefined mechanism, which eventually results in the extracellular accumulation of PrPsc aggregates. Studies were shown that cellular prion protein is present in cancer cell lines and may protect cells from pro-apoptotic agent or sensitizes cells to apoptotic stimulus. Therefore, the main objective of this study was to establish stable cell lines overexpressing prion protein in human breast cancer cell line, MCF-7 and human neuroblastoma cell line, SH-SY5Y cell lines by transfection of cDNA into MCF-7 and SH-SY5Y cell lines using the electroporation technique.

O autorze

Khor Heng Wei is a writer for Lambert Academic Publishing House. She has graduated from University Tunku Abdul Rahman holding a Bachelor's Degree in Biomedical Science in 2010 and has finished her Master's studies in Health Toxicology with University Science Malaysia in 2011.