Enzyme Nanocarriers

ISBN-13: 9789814613422 / Angielski / Twarda / 2015 / 266 str.

Daniela Cardinale; Thierry Michon

Opis

Enzyme immobilization on solid supports has been considered for a long time as an attractive solution to perform sophisticated organic synthesis, as required in the preparation of fine pharmaceutical chemicals. But in the late 1990s, the tremendous progress in molecular biology fundamentals opened up the possibilities to feed a tool box for building new bio-inspired nanotechnologies. Among them was the goal of repositioning biocatalysts in environments mimicking their genuine working place: the cell.

The research presented in this book was selected among the most impressive achievements in the fields of enzyme bio-conjugation and bio-inspired nanosupports. It opens up potential applications in nanocatalysis and for lab-on-a-chip and biosensor devices, drug delivery vectors, and nanometrology. The book is a rich source of inspiration for the researcher seeking to build smart materials requiring nanoscale positional control of functional proteins on various carriers. Most of the supports described pertain to soft materials (cells, virus, polymers, DNA) and most of the examples benefit from the amazing properties of proteins and DNA to self-assemble, according to the "bottom-up law," a specific feature of all living systems. But as treated in the last chapter, these enzyme nanocarriers have also the potential to be grafted on solid supports through "top-down" technologies, spanning orders of magnitude from the nano- to the mesoscale and above.

Recenzje

"A timely and well-presented treatise on the evolving use of biological assemblies for nanocarriers of enzymes with potential applications in catalysis and the delivery of enzyme therapeutics. In seven chapters the expert authors describe the utility of viruses, polymersomes and DNA origamis. The book will be of great value not only to the specialist but also to others with a general interest in the broader field of bionanotechnology."
— Prof. David J. Evans, University of Hull, UK

Spis treści

Virus diversity to explore various kinds of enzyme nanocarriers. Strategies for virus coupling of enzymes. Viruses as model nanoreactors to study enzyme. Nanoscale compartmentalization techniques in cascade catalysis. Nanocarriers for Therapeutic Enzymes. DNA-origamis as protein. Nanopatterning for nanobiotechnologies: emerging methods based on soft-lithography and directed.