Synthesis and Evaluation of Targeted Prodrugs for Cancer Treatment

ISBN-13: 9783847339373 / Angielski / Miękka / 164 str.

Sreeja Jayant

Opis

The Drug delivery system (DDS) consisted of several combination's of the anticancer drug doxorubicin (DOX), polymeric drug carrier polyethylene glycol (PEG), drug spacer citric acid (CA) and targeting moiety sialic acid (SA) which was evaluated in vitro for cytotoxicity on human ovarian carcinoma cells (A2780). The targeted prodrug conjugates with two copies of SA and DOX showed enhanced cytotoxicity when compared with non-targeted prodrugs and free DOX. Targeting of the conjugate to cancer cells by sialic acid and an increase in number of copies of targeting moiety and anticancer drug enhanced prodrug uptake by cancer cells and cytotoxicity of the prodrug. Analysis of synthesized conjugates by 1NMR, MALDI/TOF, and HPLC showed the formation of the PEG prodrugs. More than 40% of the drug was released from its conjugate in presence of esterase enzyme, whereas the conjugate was stable at pH 7.4. The data obtained suggest that PAMAM G4-SA dendrimer represents a promising vehicle for intracellular delivery of low solubility drugs such as paclitaxel. Developed dendrimer provides both cytoplasmic and nuclear delivery of therapeutics and enhances anticancer activity of paclitaxel.

O autorze

Completed her M.S. in Pharmaceutical Sciences from Rutgers, The State University of New Jersey, USA in 2007. Earlier, completed her Bachelor of Pharmacy from DSTSM College of Pharmacy, Solapur, Shivaji University.Her research involved molecular modeling, design, and synthesis of polymeric pro-drug conjugates for cancer targets.