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In plant biotechnology, the in-vitro studies are performed by culturing the plant cell, tissue and organ on an artificially prepared nutrient medium static or liquid under aseptic conditions. The effect of some plant growth regulators were studied on seedling growth and stomatal characters of Brassica campestris L. V.mungo (L.) Hepper, V.radiate (L.) Wilczek.) through in-vitro studies. Black gram (Vigna mungo), is an important short duration pulse crop grown in many parts of India, cultivating both in Kharif and Rabi season. The optimum temperature for better growth of these crops ranges...