Genome - Kinome : Utilize cross species genomic information to develop a species specific tool for global kinome analysis

ISBN-13: 9783639137774 / Angielski / Miękka / 2009 / 132 str.

Shakiba Jalal

Opis

Kinases belong to one of the most biologicallysignificant enzyme classes. The development ofanalytical techniques for characterization of kinaseactivity, in particular at a global scale, is acentral priority for proteomic and cell biologyresearchers.In order to facilitate global analysis of cellularphosphorylation, a new paradigm of microarraytechnology which focuses on analysis of totalcellular kinase activity, kinome, has emerged in thepast few years.As the specificity of many kinases is dictatedprimarily by recognition of residues immediatelysurrounding the site of phosphorylation a logicalmethodology is to employ peptidesrepresenting these immediate sequences asexperimental substrates. Microarray chips carryinghundreds of such substrate targets have beendeveloped for human kinome analysis, however,lack of similar tools for species outside researchmainstream has limited kinome analysis inthese species. This work describes a methodology tocreate species specific peptide arrays. A case studyis carried out to analyze Toll Like Receptor (TLR)signaling pathways in bovine monocytes.

O autorze

Shakiba Jalal has recently completed her Master of Science in
Biochemistry from the University of Saskatchewan(U of S), Canada.
She has a Bachelor of Science in Computer Science from the U of
S. Her work is published in January 2009 issue of Journal of
Science Signaling. She is now working in the Department of
Computer Science at the U of S.