Guide to Clinical and Diagnostic Virology

ISBN-13: 9781555819910 / Angielski / Miękka / 2019 / 460 str.

Reeti Khare

Spis treści

PrefaceAcknowledgmentsAbbreviationsAbout the AuthorSection I: Foundations of Clinical Virology1 Introduction to VirusesVirus structure, life cycle, Baltimore classification, figures of DNA and RNA virus classification, transmission, nomenclature2 Laboratory Diagnosis of Viral InfectionsDifferential diagnosis for viral syndromes, specimen collection, comparison of diagnostic techniquesSection II: Viral Pathogens and Clinical Presentation3 Respiratory VirusesInfluenza virus, respiratory syncytial virus, human metapneumonvirus, rhinovirus, coronavirus, mumps virus, diagram of seasonality4 Viruses with Dermatologic ManifestationsHerpes simplex virus, varicella-zoster virus, measles virus, rubella virus, human herpesviruses 6 and 7, molluscum contagiosum, smallpox virus, figure of viral rashes, description and comparison of herpesviruses 1 to -85 Gastrointestinal and Fecal-Oral Hepatitis VirusesRotavirus, norovirus, astrovirus, sapovirus, HAV, HEV, diagram of seasonality6 Viruses That Can Cause Multiple SyndromesEnterovirus and parechovirus, adenovirus, parvovirus B19, human bocavirus7 Opportunistic, Transplant-Associated VirusesCytomegalovirus, BK virus, JC virus8 Blood-Borne Hepatitis VirusesHepatitis B, C, and D viruses, and comparison of hepatitis viruses A through E9 Human RetrovirusesHIV, HTLV-1/-210 Oncogenic VirusesHPV, EBV, HHV-811 Zoonotic VirusesRabies virus, Ebola virus, Marburg virus, Lassa virus, Crimean Congo hemorrhagic virus, hantavirus, lymphochoriomeningitis virus, monkeypox virus, herpes B virus, Hendra virus, Nipah virus, comparison of zoonotic viruses12 ArbovirusesMosquitoes, ticks, dengue virus, yellow fever virus, chikungunya virus, West Nile virus, eastern, western and Venezuelan equine encephalitis viruses, Japanese encephalitis virus, Powassan virus, comparison of arbovirusesSection III: Diagnostic Assays and Techniques13 Culture- and Tissue-Based Diagnostic TechniquesConventional viral culture and cell lines, viral growth rates, hemagglutination, quantification of plaques, histology/cytology, histologic and cytologic images of virally infected cells14 Diagnostic Techniques Based on Immunological InteractionsKinetics of immune responses, interpreting serologic results, antigen and antibody detection, ELISA and chemiluminescent immunoassay, immunohistochemistry, immunoblots, immunofluorescence, lateral-flow assay, comparison of immunologic assays15 Molecular Techniques: Nucleic Acid AmplificationBasics of nucleic acids, nucleic acid amplification, PCR, RT-PCR, real-time PCR, quantitative PCR, melt curve analysis, viral loads, droplet digital PCR, nested PCR, multiplex PCR, transcription-mediated amplification, controls, contamination16 Molecular Techniques: SequencingSequencing terminology, applications, procedures, platforms (Sanger, NGS, Illumina, SMRT, Ion Torrent), data analysis, comparison of platformsSection IV: Prevention and Management of Viral Infections17 BiosafetyBiosafety levels, select agents, reportable diseases, PPE, techniques, biosafety cabinets, isolation precautions18 VaccinesActive and passive immunity, intravenous immunoglobulin, types of vaccines, delivery, complications, antibody dependent enhancement, table of available viral and other vaccines, diagram of routine vaccination schedule19 AntiviralsAntivirals against herpesviruses, influenza virus, RSV, hepatitis C, other compounds, antiretrovirals and antivirals with broad coverage, mechanisms of actionSection V: The Regulatory Environment for Laboratory Testing20 Regulatory RequirementsClassification of assays by the FDA, test complexity, regulatory agencies, CLIA, inspections, proficiency testing, billing and coding21 Assay Performance and InterpretationValidation/verification, performance characteristics (precision, accuracy, reportable range, reference range), diagnostic, clinical and analytic sensitivity and specificity, prevalence, predictive value, ROC curvesReferencesAnswersIndex