Neurohistology and Imaging Techniques

ISBN-13: 9781071604267 / Angielski / Twarda / 2020 / 472 str.

Radek Pelc; Wolfgang Walz; Ronald Doucette

Spis treści

Obituary...

Preface to the Series...

Preface...

Table of Contents...

Contributing Authors...

1 Neurohistology with a Touch of History: Technology-Driven Research

Floris G. Wouterlood and Thomas P. Langer

2 Fixation Protocols for Neurohistology: Neurons to Genes 

Elliott J. Mufson, Sylvia E. Perez, Christy M. Kelley, Melissa J. Alldred, and Stephen D. Ginsberg

3 Three-Dimensional Atlases of Insect Brains

Basil el Jundi and Stanley Heinze

4 Neuroanatomical Tracing Based on Selective Fluorochrome Expression in Transgenic animals

Floris G. Wouterlood

5 Optical Imaging Probes for Amyloid Diseases in Brain

Pratyush Kumar Mishra, Myeong-Gyun Kang, Hyun-Woo Rhee

6 Chemical Clearing of GFP-Expressing Neural Tissues

Klaus Becker, Saiedeh Saghafi, Christian Hahn, Nina Jährling, and Hans­Ulrich Dodt

7 The Properties of Light Governing Biological Microscopy

Pina Colarusso

8 Beyond Brightfield: “Forgotten” Microscopic Modalities

Radek Pelc

9 Stereomicroscopy in Neuroanatomy

Erin E. Wilson, William Chambers, Radek Pelc, Paul Nothnagle, and Michael W. Davidson

10 Conventional, Apodized, and Relief Phase-Contrast Microscopy

Radek Pelc, Zdeněk Hostounský, Tatsuro Otaki, and Kaoru Katoh

11 Ultramicroscopy of Nerve Fibers and Neurons: Fine-Tuning the Light Sheets

Saiedeh Saghafi, Klaus Becker, Nina Jährling, Christian Hahn, and Hans­Ulrich Dodt

12 Imaging and Electrophysiology of Individual Neurites Functionally Isolated in Microchannels

Heinz D. Wanzenboeck, Petra Scholze, and Johann K. Mika

13 Consumer Versus Dedicated Digital Cameras in Photomicrography

Jörg Piper and Radek Pelc

14 Digital Micrographs in Pathology

Roger S. Riley, Jorge Almenara, and Christine E. Fuller

Subject Index List ... 

Blurb

This volume explores major light microscopic imaging modalities that can be used to view nervous tissue, and discusses the steps needed to use each of them, and ways to interpret the data. The chapters in this book cover topics such as atlasing of insect brain; neuroanatomical tracing through fluorochrome expression; fluorescent probes for amyloids; or optical clearing for ultramicroscopy of GFP-expressing tissues. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Authoritative and cutting-edge, Neurohistology and Imaging Techniques is a valuable resource for both expert and novice users of major light microscopic imaging techniques, and those interested in exploring alternate imaging tools.