Stimulation and Inhibition of Neurons

ISBN-13: 9781493962686 / Angielski / Miękka / 2016 / 319 str.

Paul M. Pilowsky; Melissa M. J. Farnham; Angelina Y. Fong

Opis

This Neuromethods series book reviews methods for generating lesions and for stimulating and inhibiting neural pathways, covering new techniques such as optogenetics and the use of the in situ perfused preparation and reassessing well-known approaches.

Recenzje

From the book reviews:

"This is a neurophysiology laboratory manual with step by step instructions on how to record from preparations in vivo and in situ using the rat. ... This is a timely and excellent contribution to neurophysiology methods of great interest for students, postdocs, experienced investigators, physiologists, and neuropharmacologists." (Joseph J. Grenier, Amazon.com, March, 2015)

Spis treści

Part I: Novel Experimental Techniques

 

 

 

1. Optogenetic Stimulation of Neurons in the Brainstem of Freely Behaving Rats and Mice

 

            Stephen Abbott

 

 

 

2. Stimulating Peripheral Afferents to Evoke Cardiorespiratory Reflex responses in the In Situ Arterially-Perfused Preparation

 

            Angelina Y. Fong

 

 

 

3. Excitatory Responses to Microinjection of Glutamate Depend on Dose not Volume: A Meta-Analysis of Studies in Rat RVLM

 

            Andrea H. Gaede and Paul M. Pilowsky

 

 

 

4. Acute Activation and Inhibition of the Sympathetic Baroreceptor Reflex

 

            Melissa M.J. Farnham

 

 

 

Part II: Intracellular Signalling

 

 

 

5. Pharmacological Dissection of G Protein-Mediated Second Messenger Cascades in Neurons

 

            Mark C. Bellingham

 

 

 

6. In Vivo Manipulation of Intracellular Signalling Pathways

 

            V.J. Tallapragada

 

 

 

Part III: The Single Neurons

 

 

 

7. Identification of Spinally Projecting Neurons in the Rostral Ventrolateral Medulla In Vivo

 

            Simon McMullan

 

 

 

8. Microionotophoretic Study of Individual Neurons During Intracellular Recording

 

            Qi-Jian Sun and Paul M. Pilowsky

 

 

 

9. Neurobiotin Electroporation for Combined Structural and Functional Analysis of Neurons in Developing Mouse Brain Slices

 

            Refik Kanjhan and Mark C. Bellingham

 

 

 

10. Juxtacellular Neuronal Labelling, Physiological Characterization, and Phenotypic Identification of Single Neurons In Vivo

 

Anthony J.M. Verberne and Ida J. Llewellyn-Smith

 

 

 

Part IV: Methods for Analysis

 

 

 

11. Analysis of Sympathetic Nerve Activity Analysis of Sympathetic Nerve Activity

 

            Rohit Ramchandra

 

 

 

12. Insight into Autonomic Nervous System Control of Heart Rate in the Rat Using Analysis of Heart Rate Variability and Baroreflex Sensitivity

 

            Cara M. Hildreth, Ann K. Goodchild, and Jacqueline K. Phillips

 

 

 

13. Neurophysiological Recording of the Compound Muscle Action Potential for Motor Unit Number Estimation in Mice

 

            Shyuan T. Ngo and Mark C. Bellingham

 

 

 

Part V: Clinical Focus and Application

 

 

 

14. Animal Models of Neuropathic Pain Due to Nerve Injury

 

            Paul J. Austin and Gila Moalem-Taylor

 

 

 

15. Detection of Sensitized Nerve Responses: Dorsal Root Reflexes, Live Cell Calcium, and ROS Imaging

 

            Karin N. Westlund and Liping Zhang

 

 

 

16. Visual Evoked Potential Recording in Rodents

 

            Yuyi You, Alexander Klistorner, and Stuart L. Graham

 

 

 

17. The Visual Evoked Potential in Humans

 

            Stuart L. Graham and Alexander Klistorner

 

 

 

18. Improving Motor Activation Patterns after Stroke with Wii-Based Movement Therapy

 

            Angelica G. Thompson-Butel, Sarah E. Scheuer, and Penelope A. McNulty

Blurb

Activation, inhibition, or destruction of the nervous system or its component parts as a vital tool for the investigation of function has undergone remarkable development; indeed, new approaches have been developed that allow for these actions to be used as therapeutic tools.  In Stimulation and Inhibition of Neurons, experts in the field provide an overview of modern methods for generating lesions as well as for stimulating and inhibiting neural pathways.  Many new techniques such as optogenetics and the use of the in situ perfused preparation are examined, while, in other sections, the use and validity of more well-known approaches are reassessed. Written for the Neuromethods series, chapters examine their respective topics thoroughly and include the kind of detail and implementation advice that ensures successful results in the laboratory.

 

Authoritative and cutting-edge, Stimulation and Inhibition of Neurons serves as an ideal guide for researchers seeking to gain further knowledge of the complex functions of the brain.