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A variety of powerful techniques for monitoring and analysing events during signal transduction at the single cell level are described in this lab manual. An introductionary section on cell handling includes guidelines for constructing a perfusion chamber. A main section of the book presents protocols on fluorescence techniques such as flow cytometry, microfluorescence, ion imaging and confocal microscopy. The electrophysiological section illustrates multiple applications of the patch-clamp technique in various cell types from both animals and plants. Emphasis is put on calibration and...

Of all fluctuations in economic activity, the long wave or Kondratieff cycle is easily the most puzzling and least understood one. Does it really exist, and if so, is it only a cycle in prices or a cycle in economic activity at large? What causes it, and has it been confined to Europe or does it affect the world economy as a whole? These questions, which seemed of little relevance in the prosperous years of the postwar growth era, have gained new importance since 1973. With the downturn of the long wave, interest in it has enjoyed a revival, as it did in the 1930s. A great number of...

En 2014, Ellen V, Neerlandaise vivant en France depuis 25 ans, vient d'obtenir la fameuse Green Card. Sans hesitation elle decide de s'installer avec sa famille aux Etats-Unis. Un projet d'installation a l'etranger est forcement un grand changement et qui se prepare. Dans ce livre Ellen V partage son experience d'expatriation dans des articles et fiches pratiques Organiser et preparer son depart pour les Etats Unis est tres important, c'est la cle d'une bonne integration personnelle et professionnelle. Trouver les bons partenaires, comparer les devis demenageurs, assurances, les taux de...