Ctenophores: Methods and Protocols

ISBN-13: 9781071636411 / Angielski

Leonid L. Moroz

Spis treści

1. Brief History of Ctenophora

            Leonid L. Moroz

 

2. Ctenophora: Illustrated Guide and Taxonomy

            Leonid L. Moroz, Richard Collins, and Gustav Pauley

 

3. Brief History of Placozoa

            Daria Y. Romanova and Leonid L. Moroz

 

4. Stable Laboratory Culture System for the Ctenophore Mnemiopsis leidyi​

            Joan J. Soto-Angel, Eva-Lena Nordmann, Daniela Sturm, Maria Sachkova, Kevin Pang, and Pawel Burkhardt

 

5. Illustrated Neuroanatomy of Ctenophores: Immunohistochemistry

            Tigran P. Norekian and Leonid L. Moroz

 

6. Scanning Electron Microscopy of Ctenophores: Illustrative Atlas

            Tigran P. Norekian and Leonid L. Moroz

 

7. DNA Isolation Long-Read Genomic Sequencing in Ctenophores

            David Moraga Amador, Andrea B. Kohn, Yelena Bobkova, Nedka G. Panayotova, and Leonid L. Moroz

 

8. RNA Isolation from Ctenophores

            Andrea B. Kohn, Yelena Bobkova, and Leonid L. Moroz

 

9. Gene Expression Patterns in the Ctenophore Pleurobrachia bachei: In Situ Hybridization

            Andrea B. Kohn, Yelena Bobkova, and Leonid L. Moroz

 

10. Characterization of the Mitochondrial Proteome in the Ctenophore Mnemiopsis leidyi Using MitoPredictor

            Viraj Muthye and Dennis V. Lavrov

 

11. Expression and Functional Analysis of Ctenophore Glutamate Receptor Genes

            Mark L. Mayer

 

12. Expression, Purification, and Determination of Sensitivity to Calcium Ions of Ctenophore Photoproteins

            Lyudmila P. Burakova, Svetlana V. Markova, Natalia P. Malikova, and Eugene S. Vysotski

 

13. Functional Screening of cDNA Expression Library for Novel Ctenophore Photoproteins

            Svetlana V. Markova and Eugene S. Vysotski

 

14. Recording Cilia Activity in Ctenophores

            Tigran P. Norekian and Leonid L. Moroz

 

15. Electrophysiology of Ctenophore Smooth Muscle

            Robert W. Meech, André Bilbaut, and Mari-Luz Hernandez-Nicaise

 

16. Gap Junctions in Ctenophora

            Andrea B. Kohn and Leonid L. Moroz

 

17. Analysis and Visualization of Single-Cell Sequencing Data with Scanpy and MetaCell: A Tutorial

            Yanjun Li, Chaoyue Sun, Daria Y. Romanova, Dapeng O. Wu, Ruogu Fang, and Leonid L. Moroz

 

18. DNA Methylation in Ctenophores

            Emily C. Dabe, Andrea B. Kohn, and Leonid L. Moroz

 

19. Ocean to Tree: Leveraging Single-Molecule RNA-Seq to Repair Genome Gene Models and Improve Phylogenomic Analysis of Gene and Species Evolution

            Jan Hsiao, Lola Chenxi Deng, Leonid L. Moroz, Sreekanth H. Chalasani, and Eric Edsinger

 

20. Parallel Evolution of Transcription Factors in Basal Metazoans

            Krishanu Mukherjee and Leonid L. Moroz

 

21. Long-Term Culturing of Placozoans (Trichoplax and Hoilungia)

            Daria Y. Romanova, Frédérique Varoqueaux, Michael Eitel, Masa-aki Yoshida, Mikhail A. Nikitin, and Leonid L. Moroz

 

22. Bioinformatic Prohormone Discovery in Basal Metazoans: Insights from Trichoplax

            Mikhail A. Nikitin, Daria Y. Romanova, and Leonid L. Moroz

Blurb

This volume explores emerging approaches and methods inspired by recent research using ctenophores or placozoans, facilitating the study of these enigmatic species and ancient animal lineages. Practical protocols are integrated with introductory chapters about general biology, summarizing the diversity, evolution, genomics, development, and neurobiology of these organisms. Special chapters outline strategies and applications for culturing, microscopy, physiology, electrical synapses, and bioluminescence, molecular and single-cell biology, transcription factors, as well as gene and protein expression analyses. The book also contains bioinformatics and computational chapters covering peptidomics and epigenomics. Written for the highly successful Methods in Molecular Biology series, chapters include detailed implementation advice that leads to dependable results in the laboratory. 

Authoritative and practical, Ctenophores: Methods and Protocols is an ideal resource for comparative and experimental biologists and interdisciplinary scientists aiming to decipher fundamental principles of animal organization, origin, and the evolution of biological complexity in general, opening novel avenues for future synthetic biology research.