Cell Proliferation and Cytogenesis in the Mouse Hippocampus

ISBN-13: 9783540536895 / Angielski / Miękka / 1991 / 83 str.

Kyrill Yu Reznikov

Opis

In the present work, processes of cell proliferation, cell death, neurogenesis, and gliogenesis in the mouse hippocampus were studied. The mapping of distribution of hippocampal mitoses and counting of their number allowed a more precise definition of the data concerning the disposition and age reduction of proliferative sites in Ammon's horn and the dentate gyrus in the mouse. As a result, the following generalized scheme of development and age reduction of the germinal zones in the mouse hippocampus has been suggested. 1. Ammon's horn a) The ventricular zone, from the beginning of formation of the hippocampus (Ell) until E20 b) The suprafimbrial zone, from El6 until P7 2. Dentate gyrus a) The prime germinal zone ("the anlage of the dentate gyrus" of Stanfield and CowanI979b), from E15 until P3 b) The proliferative zone of the hilus, from P3 until Pl4 c) The subgranular zone, from P3 until adult age The adduced scheme needs some comments: 1. In the hippocampus (as well as in other formations of the developing brain), primary precursors of all types of cells of neuroectodermal origin are represented by cells of the ventricular zone. They give rise to cells of secondary germinal zones in the dentate gyrus and Ammon's horn and are direct precursors of the majority (if not of all) neuronal cells in Ammon's horn, the earliest originating generations of neurons in the dentate gyrus, hippocampal radial glial cells, and, evidently, of a considerable part of astroblasts and oligodendroblasts in Ammon's horn.

Spis treści

1 Hippocampal Formation in the Mouse and Rat — Structural Organization and Development: A Review.- 1.1 Structural Organization of the Hippocampus.- 1.1.1 General Ideas on the Hippocampus, Nomenclature, and Topography.- 1.1.2 Structural Organization of Ammon’s Horn and the Dentate Gyrus.- 1.1.3 Afferent, Efferent, and Longer Intrinsic Connections in the Hippocampus.- 1.1.4 Functions of the Hippocampus.- 1.2 Development of the Hippocampus.- 1.2.1 Neurogenesis and Gliogenesis in the Hippocampus.- 1.2.2 Differentiation in the Hippocampus.- 1.3 Conclusion.- 2 Materials and Methods.- 2.1 Animals, Histological Procedures, and Autoradiography.- 2.2 Description of Experiments and Methods of Their Analysis.- 2.2.1 Mapping and Quantitative Analysis of Mitoses and Pyknoses in the Developing Hippocampus.- 2.2.2 Postnatal Cell Genesis and Death in the Mouse Dentate Gyrus Under Normal Conditions and Under Experimental Influences.- 2.2.3 Study of Spatiotemporal Sequences of Neurogenesis in the Hippocampus and Neocortex.- 3 Cell Proliferation and Cell Death in the Developing Hippocampus of a Mouse.- 3.1 Some Aspects of the Study of Cell Proliferation and Cell Death in the Developing Hippocampus.- 3.2 Results of the Study.- 3.2.1 Registration of Arrangement of Mitoses and Pyknoses.- 3.2.2 Quantitative Analysis of Cell Proliferation and Cell Death.- 3.3 Discussion.- 3.3.1 Cell Proliferation in the Developing Hippocampus.- 3.3.2 Cell Death in the Developing Hippocampus.- 3.4 Conclusion.- 4 Postnatal Production and Death of Cells in the Mouse Dentate Gyrus.- 4.1 The Dentate Gyrus as an Object for Experimental and Clinical Investigations.- 4.2 Cytological Characteristics of Proliferating Cells in the Dentate Gyrus During Postnatal Development.- 4.3 Study of Postnatal Cytogenesis in the Dentate Gyrus.- 4.4 Kinetics of Cell Proliferation and Death in the Subgranular Zone of the Dentate Gyrus in Normal and Undernourished Mice.- 4.5 Study of the Regenerative Capacity of the Dentate Gyrus.- 4.6 Conclusion.- 5 Neurogenesis in the Hippocampus and Neocortex as the Embryonic Basis for Brain Module Formation.- 5.1 Neural Modules in the Neocortex and Hippocampus.- 5.2 Sequences of Neurogenesis and Formation of Modules in the Archi- and Neocortex: Problems of Interrelationship.- 5.3 Results of the Study.- 5.3.1 Vertical Order and Mosaicism of Neurogenesis in the Neocortex.- 5.3.2 Spatial and Temporal Sequences of Neurogenesis in Field CA1 of Ammon’s Horn.- 5.4 Discussion.- 5.5 Conclusion.- 6 Summary.- References.