Intracellular Thermometry with Fluorescent Molecular Thermometers

ISBN-13: 9783527350322 / Angielski / Twarda / 2024 / 304 str.

Japan) Uchiyama Seiichi (University of Tokyo

Opis

Understand a vital new bioanalytical technique with this comprehensive introduction to measuring temperature on the cellular scale Most organisms have highly controlled body temperatures, fluctuations in which are therefore sensitive indicators of changes in body function. In recent years, the development of fluorescent molecular thermometers and related intracellular temperature probes has enabled researchers to track these fluctuations at the cellular rather than the organismic level, opening up a whole new field of study in cell and molecular biology. Intracellular Thermometry with Fluorescent Molecular Thermometers provides bioanalytical researchers with an introduction to these technologies and their current and future applications. Starting off with a discussion of temperature as a key factor in biological regulation, it provides an authoritative overview of available fluorescent temperature probes, their characteristics and potential applications. Intracellular Thermometry with Fluorescent Molecular Thermometers readers will also find: Step by step instructions for constructing an intracellular thermometry experiment and validating results Comprehensive discussion of existing applications A vision for the future development of thermal biology as an independent discipline Authored by a pioneer in the field of intracellular thermometry, Intracellular Thermometry with Fluorescent Molecular Thermometers is ideal for researchers in analytical chemistry, cell biology, molecular biology, biophysics, or any related subjects.

Spis treści

1 Temperature in Biological Species1.1 Temperature in Our Life1.2 Body Temperature1.3 Effects of Environmental Temperature on Biological Function1.4 Molecules Associated with Biological Responses to Environmental Temperature2 Development of Fluorescent Molecular Thermometers2.1 Physical Chemistry of Fluorescence2.2 Fluorescent Molecular Thermometers2.2.1 Small Organic compounds involving intersystem crossing2.2.2 Small Organic Compounds Involving Conformational Change2.2.2 Organometallic Complexes2.2.3 Excimers and Exciplex2.2.4 Host-Guest Complexes2.2.5 Thermoresponsive Macromolecules2.2.6 Inorganic Materials2.2.7 Others3 Intracellular Thermometry with Fluorescent Molecular Thermometers3.1 Early Attempts for Cellular Thermometry 3.2 Introduction of Fluorescent Molecular Thermometers into a Living Cell 3.3 Cytotoxicity Assessment3.4 Practice of Intracellular Thermometry with Fluorescent Molecular Thermometers3.5 Intracellular Thermometry with Organelle-targeted Fluorescent Molecular Thermometers3.6 Intracellular Thermometry of Brown Adipocytes3.7 Application of Intracellular Thermometry in Various Biological Fields4 Cellular Thermometry Based on Non-fluorometric Principles4.1 Infrared Thermometry 4.2 Photoacoustic Thermometry 4.3 Raman Thermometry 4.4 Use of Transmission Spectroscopy 4.5 Thermocouples4.6 Resonant Thermal Sensors4.7 Bimaterial Microcantilevers4.8 Thermistors5 Reliability of Intracellular Thermometry with Fluorescent Molecular Thermometers5.1 Validity of a Calibration Curve 5.2 Possible Artifacts in Thermometry of Biological Samples5.3 Difference between Experimentally Observed and Theoretically Calculated Intracellular Temperature Variation6 The Future of Intracellular Thermometry6.1 In vivo Thermometry6.2 Thermal Medicine6.3 Thermal Signaling6.4 Absolute Determination of Intracellular Temperature6.5 Simultaneous Measurement of Multiple Parameters6.6 Intracellular Thermometry of Plant CellsAppendixA.1 Review and account items on fluorescent molecular thermometersA.2 Comprehensive collection of fluorescent polymeric thermometers based on the combination of a thermoresponsive polymer and an environment-sensitive fluorophoreA.3 Comprehensive collection of fluorescent nanogel thermometers based on the combination of a thermoresponsive polymer and an environment-sensitive fluorophoreA.4 Non-covalent mixtures of a thermoresponsive polymer and an environment-sensitive fluorophoreA.5 Fluorescent logic gates based on the combination of a thermoresponsive polymer and an environment-sensitive fluorophoreA.6 Fluorescent inorganic thermometers

O autorze

Seiichi Uchiyama has been an Assistant Professor at the University of Tokyo since 2005. After receiving his academic degrees in Pharmacy from the University of Tokyo with Prof. Kazuhiro Imai he spent three years as a postdoctoral researcher at Nara Women's University with Prof. Kaoru Iwai and at Queen's University of Belfast with Prof. A. Prasanna de Silva. After returning to the University of Tokyo in 2005, he started his career as an experimental researcher and continues to work as an independent leader of scientific projects funded by the Japanese government. His scientific interests include analytical and photophysical chemistry and the development of fluorescent sensors based on novel functional mechanisms.