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Tetanus, although a disease of third world but significant number of cases are reported every year in America and Europe.Annual world mortality from tetanus is estimated to be 800,000- 1000,000 making it a significant public health problem in developed countries.Tetanus is characterized by muscle spasms which leads to asphyxia and ultimately death if untreated. Conventional treatment is heavy sedation to avoid spasms along with other supportive measures. Heavy sedation leads to artificial ventilation.More than 60% mortality in tetanus occurs due to heavy sedation and artificial...

Polygalacturonase one of the hydrolytic enzymes involved in the degradation of pectin and cellulose content of the growing tissues in many horticultural plants. CaPG gene is responsible for the production of polygalacturonase in capsicum cultivars, under laid project is associated with the cloning of CaPG gene and construction of many appropriate vectors carries the aim gene in bacteria and other interested commodities. Expression vector termed as pVBG2307 is unique and novel vector offering more broader cloning site with greater than twenty restriction endonucleases.