Molecular And Conventional Identification of Bacteria in Neonatal Sepsis

ISBN-13: 9783847339335 / Angielski

Hesnaa Al-Mossawi; Jabbar S. Hassan; Nada M. Al-Bashier

Opis

A total of 100 hospitalized children from zero to 30 days of age were enrolled in this cross-sectional study, the participants neonates were grouped into two categories according to type of onset. The first was early onset group included children in age groups from (0- 7 days) while other late onset group included children from (7-30 days). Blood samples were obtained from each subject participate in this study, blood samples were used in the diagnosis of neonatal infection by using conventional methods including blood culture and API system, conventional PCR based on 16rRNAN as a universal gene for diagnosis of bacterial infection after DNA extraction from serum sample. Half of positive results in the conventional PCR were sequenced for bacterial identification according to the Macrogen, Inc. South Korea public biotechnology company. The neonatal sepsis was caused by different type of bacteria; the most common cause of neonatal sepsis was Staphylococcus aureus and coagulase negative Staphylococcus of gram positive bacteria while Pseudomonas aeruginosa and Acinetobacter bumanii were the most common gram negative bacteria.

O autorze

Al-Mossawi, Hesnaa
Name: Hesnaa Saeed Mohsin Al-Mossawi, Date of Birth: 10/11/1990, July 2008: general certificate of secondary education/al Thaqafa high school, July 2013: BVMS / University of Karbala, January 2013: Regular stay in the Faculty of Veterinary Medicine, January 2018: MSc of Medical Microbiology/University of al-Nahrain.