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Salmonella: Methods and Protocols presents detailed methods on a variety of aspects of Salmonella research, focusing on those which provide landmarks for future discovery. Highlighted topics include molecular genotyping, rapid and accurate new methods for sensitive determination of antibiotic resistance profiles, the characterization of Salmonella-specific cell antigens, and Salmonella-host cell interactions. Detailed methods for studying Salmonella motility are also presented. Each chapter provides a short overview of the topic, which is followed by detailed explanations of techniques...

Major improvements in instrumentation and specimen preparation have brought SEM to the fore as a biological imaging technique. In FESEM, a field-emission cathode placed in the electron gun of a scanning electron microscope provides narrower probing beams and high electron energy. The result is improved spatial resolution and minimized sample charging and damage. Images produced are less destroyed and have a spatial resolution down to 1.5 nm, three to six times better than conventional SEM.

Although this imaging technique has undergone tremendous developments, it is still poorly...

Major improvements in instrumentation and specimen preparation have brought SEM to the fore as a biological imaging technique. In FESEM, a field-emission cathode placed in the electron gun of a scanning electron microscope provides narrower probing beams and high electron energy. The result is improved spatial resolution and minimized sample charging and damage. Images produced are less destroyed and have a spatial resolution down to 1.5 nm, three to six times better than conventional SEM.

Although this imaging technique has undergone tremendous developments, it is still poorly...