Coagulation protein FVIII binding to phospholipid membranes : investigated by Fluorescence Correlation Spectroscopy

ISBN-13: 9783838117720 / Angielski / Miękka / 2010 / 112 str.

Hanna Engelke

Opis

Dynamics and interactions of proteins and membranes are essential biological processes. Fluorescence Correlation Spectroscopy allows one to measure these dynamics and interactions quantitatively in buffer as well as in their biological environment. Here, the mechanism of coagulation factor VIII binding to membranes, a key element in the intravascular pathway of the coagulation cascade, is investigated using Fluorescence Correlation Spectroscopy. The interplay of factor VIII binding with competing proteins is studied on the example of annexin V. The latter has a very efficient regulatory influence on factor VIII binding, which is modeled quantitatively. The natural environment can strongly influence biological processes, such as coagulation. Comparison of experiments on factor VIII binding in buffer and in plasma elucidates such influences. Furthermore, the dynamics of the actin-cytoskeleton of living cells is studied and a drug delivery system based on lipid-coated nanoparticles is described and characterized.

O autorze

Hanna Engelke studied physics at the Universität Bayreuth andgraduated in 2005. She received her doctoral degree in 2010 at theLudwig-Maximilians-Universität München.